Protesters are still occupying a main crossing at Canada’s border with the United States, despite a court ruling ordering them to leave.
The injunction came into effect at 19:00 local time (midnight GMT) on Friday to end days of protests.
Truckers are blockading Ambassador Bridge, a vital trade link between Windsor, Ontario and Detroit, Michigan.
Truckers’ protests against Covid restrictions are also ongoing at other border crossings and in Ottawa.
The injunction was filed by the city of Windsor and the Automotive Parts Manufacturers’ Association, which argued that they were losing as much as $50m ($39m; £29m) per day because of the convoy.
Following the court order, Windsor Police put out a statement to “make demonstrators clearly aware that it is a criminal offence” to block the border crossing. The police added that a criminal conviction could lead to the seizure of vehicles and the inability to enter the US.
On Friday, Prime Minister Justin Trudeau spoke with US President Joe Biden about the border blockades.
The pair discussed their “shared challenges at the border”, Mr Trudeau said, with the prime minister promising quick action to resume trade.
Mr Trudeau added that he and Mr Biden also discussed US phone calls that have flooded police emergency call centres in Canada, and the donations and assistance that protesters are getting from American citizens.
BBC